Characterization of white spot syndrome virus immediate-early gene promoters
نویسندگان
چکیده
منابع مشابه
Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1.
Immediate-early proteins from many viruses function as transcriptional regulators and exhibit transactivation activity, DNA binding activity, and dimerization. In this study, we investigated these characteristics in white spot syndrome virus (WSSV) immediate-early protein 1 (IE1) and attempted to map the corresponding functional domains. Transactivation was investigated by transiently expressin...
متن کاملWhite spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1.
Although the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway is part of the antiviral response in arthropods such as Drosophila, here we show that white spot syndrome virus (WSSV) uses a shrimp STAT as a transcription factor to enhance viral gene expression in host cells. In a series of deletion and mutation assays using the WSSV immediate-early gene i...
متن کاملGene-expression profiling of White spot syndrome virus in vivo.
White spot syndrome virus, type species of the genus Whispovirus in the family Nimaviridae, is a large, double-stranded DNA (dsDNA) virus that infects crustaceans. The genome of the completely sequenced isolate WSSV-TH encodes 184 putative open reading frames (ORFs), the functions of which are largely unknown. To study the transcription of these ORFs, a DNA microarray was constructed, containin...
متن کاملMolecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...
متن کاملMolecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2013
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.047274-0